Bethesda system for Thyroid Malignancies 2007, 2017 and 2023 Updates

Papillary Carcinoma thyroid histopathology

This article is mainly intedend for use by medical professionals.

Fine needle aspiration cytology (FNAC) is a corner stone investigation done in the evaluation of thyroid lesion. It is a simple, safe, quick and cost-effective outpatient procedure for diagnosing thyroid patholodies.

FNAC has got a high degree of diagnostic accuracy (85-100%) and a high positive predictive value for malignant diseases (94%). The results are superior when FNAC is performed under ultrasound-guidance (USFNAC).

In this procedure, a small sample of thyroid tissue is removed from the gland with the help of a 24-27G needle under local anesthesia and is looked under a microscope to identify any pathologies.

Need for a universal reporting system / Bethesda System

In order to avoid the nonuniform, ambiguous and confusing terminologies used by cytopathologists for reporting thyroid FNAC throughout the world, the National Cancer Institute (NCI) organized a “Thyroid Fine Needle Aspiration State of the Art and Science Conference” in Bethesda, Maryland in the year 2007.

In this conference, they proposed the Bethesda System for Reporting Thyroid Cytology (TBSRTC) which established a standardized, category-based reporting system for thyroid fine-needle aspiration (FNAC) specimens.

The primary purpose of terminologies used in the Bethesda system is to have a clarity of communication between the cytopathologist and the surgeon, endocrinologist or radiologist. The terminologies should provide clinically relevant information, which should have an implied (or explicit) risk of malignancy on which recommendations for patient management (eg, annual follow-up, repeated FNAC, surgical lobectomy, near-total thyroidectomy) can be based.

Various studies have shown that the Bethesda system for reporting thyroid cytology reduces interobserver variability in reporting thyroid FNACs. TBSRTC also improves communication between the cytopathologist and surgeon by way of indicating cancer risk in each category. In addition, it also provides guidelines for management and allows easy and reliable sharing of data between different laboratories.

2007 Bethesda – 1 system

The 2007 Bethesda system recommends six general diagnostic categories and suggests that each report should begin with a general diagnostic category. Each of the categories has an implied cancer risk (ranging from 0 to 3% for the benign category to nearly 100% for the malignant category) with rational clinical management guidelines.

Category I — Nondiagnostic or unsatisfactory (ND/UNS) : This group involves specimens showing nonspecific features not conclusively diagnostic of a particular entity. These include cyst fluid only, virtually acellular specimens or others like obscuring blood, clotting artifact, etc. In regular practice, this should be ideally limited to no more than 10% of total thyroid FNACs.

Category II — Benign : This category includes benign follicular nodule (colloid nodule, adenomatoid nodule), lymphocytic (Hashimoto’s) thyroiditis and granulomatous (subacute) thyroiditis.

Category III — Atypia of undetermined significance (AUS) or follicular lesion of undetermined significance (FLUS) : In this category, FNAC specimens that do not fit into benign, suspicious or malignant categories are included. This terminology is reserved for specimens containing cells (lymphoid, follicular) with architectural atypia which is not sufficient to be classified as suspicious for a follicular neoplasm (FN) or malignancy and on the other hand atypia is more marked than benign change. The pathologist should specify if it is Hurthle type or Oncocytic type.

Category IV — Follicular neoplasm (FN) or suspicious for a Follicular neoplasm (FN/SFN) : In this category, specimens with significant alteration in the follicular cell architecture, characterized by cell crowding, micro follicles, dispersed isolated cells and scant or absent colloid are included.

Category V — Suspicious for malignancy : This category includes lesions suspicious for papillary carcinoma, medullary carcinoma, other malignancies (eg, lymphoma, metastatic carcinomas), or neoplasm because of total necrosis of lesional cells (e.g., anaplastic carcinoma).

Category VI — Malignant lesion: This category includes specimens with cytomorphologic features which are conclusive for malignancy.

After its introduction in 2007, the Bethesda system got widespread acceptance, especially in the U.S., The American Thyroid Association (ATA) endorsed the system officially in 2015 as part of their revised ATA guidelines and risk stratification. TBSRTC is now the most common classification worldwide for the reporting of thyroid FNAC specimen.

The new advances in the field like Noninvasive Follicular Thyroid Neoplasm with Papillary-Like Nuclear Features (NIFTP), the utility of molecular and IHC markers, and clinical management advancements has necessitated a need for revision of the TBSRTC system. Hence, a second edition for the system was released by the International Cytology Congress, in a symposium held during its meeting in Yokohama, Japan, in 2016.

2017 Bethesda – 2 system for thyroid

Following are the highlights in 2017 revised Bethesda system.

  • Every thyroid FNAC report should begin with one of six diagnostic categories, the names of which remain unchanged since they were first introduced:
    • nondiagnostic or unsatisfactory
    • benign
    • atypia of undetermined significance (AUS) or follicular lesion of undetermined significance (FLUS)
    • follicular neoplasm or suspicious for a follicular neoplasm
    • suspicious for malignancy
    • malignant
  • There is a choice of two different names for some of the categories. A laboratory should choose the one it prefers and use it exclusively for that category.
  • Synonymous terms (e.g., AUS and FLUS) should not be used to denote two distinct interpretations.
  • The malignancy risks have been updated based on new data.
  • Each category is linked to updated, evidence-based clinical management recommendations.
Comparison of Bethesda I with Bethesda II (Changes are marked in bold)
 

 

Risk of malignancy Management option
Bethesda I Bethesda II Bethesda I Bethesda II
Nondiagnostic or

Unsatisfactory

1-4% 5-10% Repeat USG FNAC Repeat USG FNAC
Benign 0-3% 0-3% Clinical and imaging follow-up Clinical and imaging follow-up
Atypia of unknown significance or

follicular lesion of undetermined significance.

5-15% 10-30% Repeat FNAC Repeat FNAC or Molecular studies/diagnostic lobectomy
Follicular neoplasm 20-30% 25-40% Surgical lobectomy Molecular studies/ diagnostic lobectomy
Suspicious of malignancy 60-75% 50-75% Lobectomy / Total thyroidectomy Total thyroidectomy/ lobectomy
Malignant 97-99% 97-99% Total thyroidectomy Total thyroidectomy/ lobectomy

2023 Update / 3rd Edition of Bethesda

The aim of the recently published third edition of TBSRTC (2023) is to provide a higher degree of granularity to the reporting system by incorporating unified terminology and eliminating duplication in diagnostic nomenclature.  It focuses on aligning the Bethesda nomenclature with the recent 2022 World Health Organization Classification of Thyroid Neoplasms, further refining the category-based risk of malignancy estimates. In addition, it introduces cytology-based subcategorization of atypia of undetermined significance (AUS) and includes focused chapters on clinical perspectives, imaging studies, and the use of molecular and other ancillary tests.

Following are the key updates within each diagnostic category follows:

Diagnostic Category TBSRTC 2023 Recommendations
Nondiagnostic
  • “Unsatisfactory” terminology is removed.
  • A repeat FNA can be performed in less than 3 months.
Benign
  • The term “follicular nodular disease” replaces previously used terminologies including colloid nodule, hyperplastic nodule, adenomatous nodule, or benign follicular nodule.
Atypia of undetermined significance (AUS)
  • The term “follicular lesion of undetermined significance” is discontinued.
  • AUS is subclassified into 2 subgroups: AUS—nuclear atypia and AUS—other.
Follicular neoplasm (FN) and follicular neoplasm–oncocytic follicular neoplasm (FN-OFN)
  • The term “suspicious for follicular neoplasm” is discontinued.
  • Follicular-patterned lesions with mild or subtle nuclear atypia (potential cases of Noninvasive Follicular Thyroid neoplasm with papillary-like nuclear features NIFTP or Follicular Variant of Papillary Carcinoma Thyroid FV-PTC) should be classified as FN and not Suspicious for malignancy (SFM).
  • “Follicular neoplasm–Hurthle cell type” replaced by “follicular neoplasm–oncocytic follicular neoplasm.”
Suspicious for malignancy (SFM)
  • The diagnosis of SFM should be used judicially (avoid categorizing NIFTP and FV-PTC as SFM).
Malignant
  • The term “papillary thyroid carcinoma, variants” is changed to “papillary thyroid carcinoma-subtypes.”
  • “Cribriform morular variant,” previously recognized as a papillary thyroid carcinoma subtype, is now designated as a separate tumor entity.
  • “Cribriform morular variant of papillary thyroid carcinoma” is now replaced with “high-grade follicular-derived thyroid carcinoma.”

Summary of 2023 Update

Bethesda System for Reporting Thyroid Cytopathology, third edition, 2023
Diagnostic Category Examples of Diagnostic Entities Range of Estimated ROM (%) Clinical Management Options
Nondiagnostic
  • Cyst fluid only
  • Virtually acellular specimen
  • Obscuring blood, clotting artifacts, drying artifact, etc.
5–20% Repeat FNA with ultrasound guidance
Benign
  • Consistent with FND (includes AN, CN, etc.)
  • Consistent with CLT in the proper clinical context
  • Consistent with granulomatous (subacute) thyroiditis
2–7% Clinical and ultrasound follow-up
Atypia of undetermined significance (AUS)
  • AUS—nuclear type
  • AUS—other
  • Overall 13–30%
  • AUS—nuclear atypia: ROM 36–44%
  • AUS—other: ROM 15–23%
Repeat FNA, molecular testing, diagnostic lobectomy, or surveillance
Follicular neoplasm (FN)
  • FN
  • FN—oncocytic type
23–34% Molecular testing, diagnostic lobectomy
Suspicious for malignancy (SFM)
  • Suspicious for PTC
  • Suspicious for MTC
  • Suspicious for metastatic carcinoma
  • Suspicious for lymphoma
67–83% Molecular testing, lobectomy, or total thyroidectomy
Malignant
  • PTC
  • HGFDCTC
  • MTC
  • ANTC
  • SCC
  • Carcinoma with mixed features (specify)
  • Metastatic malignancy
  • NHL
97–100% Lobectomy or total thyroidectomy
FND: follicular nodular disease; AN: adenomatoid nodule; CN: colloid nodule; CLT: chronic lymphocytic (Hashimoto) thyroiditis; PTC: papillary thyroid carcinoma; HGFCDTC: high-grade follicular cell-derived thyroid carcinoma; MTC: medullary thyroid carcinoma; ANTC: anaplastic carcinoma; SCC: squamous cell carcinoma; NHL: non-Hodgkin lymphoma.

References

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